The large PCR respiratory PCR panel is designed for multiplex in vitro assessment of 9 common respiratory microbiota markers, using nucleic acid amplification. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye. Positive controls included. Amplification reagents included.
- Instruments: Compatible with most Applied Biosystems and Bio-Rad real-time PCR systems.
- Dyes: FAM, HEX, Cy5, ROX.
- Multiplex: 4 targets per PCR reaction (including amplification controls). Total of three PCR reactions per panel.
- Reaction size: 15µl