Antibiotic Resistance PCR Panel – Large

The antibiotic resistance marker large PCR panel is designed for qualitative multiplex in vitro assessment of 21 common antibiotic resistant genes, using nucleic acid amplification. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye. Positive controls included. Amplification reagents included.

• Instruments: Compatible with most Applied Biosystems and Bio-Rad real-time PCR systems.
• Dyes: FAM, HEX, Cy5, ROX.
• Multiplex: 4 targets per PCR reaction (including amplification controls). Total of six PCR reactions per panel.
• Reaction size: 15µl

For Research Use Only. Not intended for in vitro diagnostics. Not approved by the FDA.

Compound: Resistance Genes

Cephalosporins: ACT, ampC, FOX

Macrolide: erm(A), erm(B)

Sulfonamide: sul1, sul2

Carbapenem: NDM-1, KPC, OXA-48, VIM, IMP-7

Methicillin: mecA

Tetracycline: tet(A)

ESBL: SHV, TEM, CTX-M group 1, CTX-M group 2, CTX-M group 9

Quinolone & fluoroquinolone: qnrB, qnrA, qnrS

Vancomycin: vanA1, vanA2, vanB