Antibiotic Resistance PCR Panel – Large

The antibiotic resistance marker large PCR panel is designed for qualitative multiplex in vitro assessment of 21 common antibiotic resistant genes, using nucleic acid amplification. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye. Positive controls included. Amplification reagents included.

  • Instruments: Compatible with most Applied Biosystems and Bio-Rad real-time PCR systems.
  • Dyes: FAM, HEX, Cy5, ROX.
  • Multiplex: 4 targets per PCR reaction (including amplification controls). Total of six PCR reactions per panel.
  • Reaction size: 15µl

 

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Targets

AmpC:

ACT, ampC, FOX 

Macrolide:

erm(A), erm(B) 

Sulfonamide:

sul1, sul2

Carbapenem:

NDM-1, KPC, OXA-48, VIM, IMP-7 

Methicillin:

mecA 

Tetracycline:

tet(A)

ESBL:

SHV, TEM, CTX-M group 1, CTX-M group 2, CTX-M group 9

Quinolone & fluoroquinolone:

qnrB, qnrA, qnrS 

Vancomycin:

vanA1, vanA2, vanB