Understanding cellular energy production starts with accurate measurement of NAD⁺ and NADH levels. These essential coenzymes play a central role in glycolysis, the Krebs cycle, and overall mitochondrial function, making them critical readouts in metabolic assays. The BlazedX NAD⁺ NADH Assay offers a robust, user-friendly solution to track these critical biomarkers—no mass spectrometry required.
| Parameter | Specification |
| Sample Type | Whole blood (heparin), 150 µl |
| Detection Method | Colorimetric (570–573 nm) |
| LOD – NAD⁺ | 0.66 µM ± 0.03 µM |
| LOD – NADH | 0.40 µM ± 0.01 µM |
| Reproducibility (CV%) | NAD⁺: 2.28–4.45%, NADH: 5.2–8.45% |
| Intra-Assay Precision | NAD⁺: 1.48 ± 0.8%, NADH: 3.33 ± 1.5% |
| Storage of Treated Samples | ≤7 days at -80°C |
| Assay Cycle Time | ~3 hours |
| Hands-On Time | ~20 minutes |
Made in the USA. Research Use Only.
The BlazedX NAD⁺ NADH Assay provides laboratories with a sensitive, scalable method to explore the biological significance of NAD fluctuations—without reliance on high-cost analytical platforms.
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